5x longamp taq reaction buffer (New England Biolabs)

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New England Biolabs 5x longamp taq reaction buffer
5x Longamp Taq Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 792 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/longamp+taq+reaction+buffer/LongAmp+Taq+DNA+Polymerase/bio_rxiv__64898__2026__03__31__715508-291-7-12
Average 98 stars, based on 792 article reviews

5x longamp taq reaction buffer - by Bioz Stars, 2026-09

98/100 stars

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Article Title: A Nanopore Sequencing-based Pharmacogenomic Panel to Personalize Tuberculosis Drug Dosing
Article Snippet: The final reaction was performed in a 50-μl volume containing 1× LongAmp Taq Reaction Buffer (NEB), 2.5 M Betaine (Sigma), and 5 U LongAmp Taq DNA Polymerase.

Article Title: A Nanopore sequencing-based pharmacogenomic panel to personalize tuberculosis drug dosing
Article Snippet: The final reaction was carried out in a 50μl volume containing 1x LongAmp Taq Reaction Buffer (NEB), 2.5M Betaine (Sigma), 5U LongAmp Taq DNA polymerase.

Polymerase Chain Reaction:

Article Title: Whole Genomic Constellation of Avian Reovirus Strains Isolated from Broilers with Arthritis in North Carolina, USA
Article Snippet: .. Briefly, a 25 μL PCR mixture was obtained using 2 μL of cDNA template, 500 nM each of the forward and reverse primer, 300 μM dNTPs, 5 μL of 5× LongAmp Taq Reaction Buffer and 1 μL (2.5 U) of LongAmp Taq DNA Polymerase (NEB, Ipswich, MA, USA). .. The PCR was performed at 94 °C for 3 min, followed by 35 cycles of 94 °C for 30 s and 65 °C for 4 min 30 s, with a final extension at 65 °C for 10 min in a Veriti TM Fast Thermal Cycler (Applied Biosystems TM , San Francisco, CA, USA, Cat. 4375305).

Article Title: Tandem duplication of a genomic region encoding glutathione S-transferase epsilon-2 and -4 genes in DDT-resistant Anopheles stephensi strain from India
Article Snippet: .. The PCR reaction contained 1X LongAmp Taq reaction buffer (New England BioLabs), 2.5 units of LongAmp Taq DNA polymerase, 300 μM dNTPs, 0.2 μM of each primer and 0.5 μl of gDNA in a total reaction volume of 25 μl. ..

Article Title: Whole Genomic Constellation of Avian Reovirus Strains Isolated from Broilers with Arthritis in North Carolina, USA.
Article Snippet: .. Briefly, a 25 μL PCR mixture was obtained using 2 μL of cDNA template, 500 nM each of the forward and reverse primer, 300 μM dNTPs, 5 μL of 5× LongAmp Taq Reaction Buffer and 1 μL (2.5 U) of LongAmp Taq DNA Polymerase (NEB, Ipswich, MA, USA). ..

Article Title: Placental methylome reveals a 22q13.33 brain regulatory gene locus associated with autism.
Article Snippet: .. A 25 μl PCR reaction mixture contained 100 ng genomics DNA, 5 μl 5× LongAmp Taq reaction buffer (NEB, Ipswich, MA, USA), 1 μl LongAmp Taq DNA polymerase (NEB, Ipswich, MA, USA), 1 μl 10 mM dNTPs, and 2 μl of 10 μM forward and reverse primer. .. The PCR amplifications were performed using following conditions: initial denaturation at 94 °C for 30 s; 30 cycles of denaturing at 94 °C for 30 s, 52 °C for 30 s, and 65 °C for 2 min with a final extension at 65 °C for 10 min. PCR products were subjected to Topoisomerase (TOPO) PCR Cloning Kit (Thermo Fisher Scientific, Waltham, MA, USA) followed by a 1.5% agarose gel electrophoresis with purification and Sanger sequencing by University of California, Davis DNA Sequencing Facility (Davis, CA, USA), and chromatograms were analyzed using SnapGene (Genewiz, South Plainfield, NJ, USA).

Nested PCR:

Article Title: Confirmatory test of active IHHNV infection in shrimp by immunohistochemistry and IHHNV-LongAmp PCR.
Article Snippet: .. For the first round of LA− nested PCR, the reaction mixture was prepared in a volume of 12.5 μL containing 1.25 μL of 5× LongAmp® Taq reaction buffer, 0.375 μL of 10 mM deoxynucleotide triphosphates (dNTPs), 0.5 μL of each primer (3665F/3665R) (Table 1), 0.5 μL LongAmp® Taq DNA polymerase (New England, BioLabs® Inc.), 2 μL of DNA template (100 ng) and 6.125 μL of DEPC water. ..

Amplification:

Article Title: Rapid targeted gene disruption in Bacillus anthracis
Article Snippet: Specific primers flanking the site of chromosomal insertion were designed and synthesized by Integrated DNA Technologies. .. DNA was amplified using 1 μL of LongAmp Taq DNA Polymerase (New England Biolabs), 2.5 μL of 5× LongAmp Taq Reaction Buffer, 0.4 μL of 10 mM dNTP Mix (New England Biolabs), nuclease-free water (Ambion), 200 nM of each primer and whole cell template in a final volume of 12.5 μL per reaction. .. The following cycling conditions were used: initial denaturation at 94°C for 30 sec, followed by 30 cycles of denaturation at 94°C for 30 sec, annealing at 60°C for 45 sec and extension at 65°C for 3 min, with a final extension at 65°C for 10 min. Products were visualized using 1% ethidium bromide (Fisher Bioreagents) on a 1% agarose gel.

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